Recombinant Alpaca anti-兔 IgG Antibody (Alexa Fluor 647)
Quick Overview for Recombinant Alpaca anti-兔 IgG Antibody (Alexa Fluor 647) (ABIN7272930)
抗原
抗体类型
片段
克隆类型
标记
应用范围
克隆位点
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适用
- 兔
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宿主
- Alpaca
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Expression System
- E.coli
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特异性
- Rabbit IgG, does not cross-react with mouse, guinea pig or chicken immunoglobulins
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纯化方法
- Produced in: E.coli
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免疫原
- This new FluoTag-X2 anti-Rabbit IgG is a species-specific FluoTag directed against IgGs from Rabbit. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications.
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Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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Guinea Pig anti-Rabbit IgG (Heavy & Light Chain) antibody - Preadsorbed
ELISA, IHC, WB, CUT&Tag, CUT&RUN Polyclonal IgG unconjugated
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (FITC)WB, FACS, FLISA, FM, DB Polyclonal IgG FITC
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)High-quality product from Rockland ELISA, IHC, WB, DB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 800)ELISA, WB, FLISA, FM, DB Polyclonal IgG DyLight 800
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP)ELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG (Heavy & Light Chain) antibody (HRP) - PreadsorbedELISA, IHC, WB Polyclonal IgG HRP
Goat anti-Rabbit IgG antibody (DyLight 488)WB, IF, FLISA, FM, DB, MA Polyclonal IgG DyLight 488
Goat anti-Rabbit IgG antibody (DyLight 680)WB, FLISA, FM, DB Polyclonal IgG DyLight 680
Goat anti-Rabbit IgG antibody (DyLight 800) - PreadsorbedWB, FLISA, FM, DB Polyclonal IgG DyLight 800
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应用备注
- 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
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缓冲液
- The single sdAb clone was lyophilized from PBS pH 7.4.
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储存条件
- -20 °C/-80 °C
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储存方法
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- IgG
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物质类
- Antibody
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背景
- Like many other mammals, the immune repertoire of rabbits consists of several immunoglobulin (Ig) isotypes (IgA, IgG, IgE, and IgM). However, compared to other species, rabbits possess only one type of IgG carrying either a lambda or kappa light chain. Historically, rabbit antibodies have been a rich source of polyclonal antibodies, which were employed in various fields of life sciences. They are used either directly from animal sera, or as affinity-purified reagents. In addition, advances in antibody engineering over the last decade enabled the generation of monoclonal rabbit antibodies. Independent of its clonality and irrespectively of its VH domain, our alpaca-derived single domain antibody (sdAb, or nanobody) binds the constant domain (Fc) of any rabbit IgG, specifically and strongly, which makes this secondary tool ideal for demanding imaging applications.
抗原
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